Large particle for clog free, superior flow in absence of a vacuum good for viscous samples such as. For a theoretical discussion of spe, and chromatographic theory in general, we suggest that the reader consult the literature references 14 in appendix iv. A nonretentive method retains and removes unwanted matrix interferences using the spe sorbent chemistry while the analytes of interest flow through the cartridge with the load fraction. Phenomenex offers this spe users guide as a practical aide for spe method development, optimization, and troubleshooting.
Contact us today for any of the traditional silica spe sorbents seen in the table below. The system performed an automated pipeline conceptual optimization, buckling and free span design, and finally conducted riser integrity and equipment integrity. Learn how to develop a customized method for spe and view. Oct 12, 2016 phenomenex, a privately held manufacturer of consumables for the separation sciences, will operate as a standalone business and will retain its brand. The spe cartridge in nonretentive spe is analogous to a chemical filter. Jun 15, 2016 a nonretentive method retains and removes unwanted matrix interferences using the spe sorbent chemistry while the analytes of interest flow through the cartridge with the load fraction.
A novel technique on how to rapidly clean up hydrolyzed urine will also be introduced. The complete guide to solid phase extraction spe abstract. Spe strata x hplc accessories bioseparations prep process gc 3 sample preparation spe stratax polymeric sorbents excellent tubetotube and welltowell reproducibility simple and fast method development high recoveries for acidic, basic, polar, and nonpolar analytes. An introduction to basic theory, method development, and applications conference paper pdf available november 2015 with 4,431 reads how we measure reads. The material does not necessarily reflect any position of the society of petroleum engineers, its officers, or members. The packed section has the same diameter like a 1 ml tube. Improved methods for forensic toxicology analysis phenomenex. Phenomenex, a privately held manufacturer of consumables for the separation sciences, will operate as a standalone business and will retain its brand. If you are unsatisfied for any reason, please give your phenomenex technical representative a call. Data use policy society of petroleum engineers spe.
Strataxa has been added to phenomenexs line of solid phase extraction spe products. Society of petroleum engineers 1079 society of petroleum evaluation engineers. The spe extracts were spiked with a mixture of fumonisin b1 and zearalenone at 2 different concentrations. Clean up and concentrate your samples with the latest sample preparation solutions from phenomenex. Danaher acquires chromatography consumables firm phenomenex.
Phenomenex offers this spe users guide as a practical aide for spe method. Sample preparation phenomenex uhplc, hplc, spe, gc. Phenomenex is a global manufacturer of uhplc, hplc columns, gc columns, sample preparation products and chromatography accessories and consumables. General rule of thumbcollect all fractions to help troubleshoot and method optimization method inconsistencies can come from. The spe data use policy is intended to provide the greatest possible access by spe members to information in the societys database.
Simplified solid phase extraction spe with stratax. Phenomenex spe vacuum manifolds are available in 10, 12 and 24position configurations. Phenomenex solid phase extraction spe sorbents are most commonly categorized by the nature of their primary interaction or retention mechanism with the analyte of interest. With the coollock nut from phenomenex, many of these problems are eliminated. Our products are designed for a broad range of sample types and applications while supporting different analyte extraction techniques and cleanup needs. Simplified solid phase extraction spe with stratax youtube. Spe reference manual and user guide phenomenex uhplc. Using liquid chromatography principles to control selectivity, spe provides the sample cleanup, recovery, and concentration necessary for accurate quantitative analysis. Solid phase extraction solid phase extraction spe is a powerful method for sample preparation and is used by most chromatographers today.
It also is prepared to be in compliance with applicable laws. For a theoretical discussion of spe, and chromatographic theory in general, we suggest that the reader consult the literature references 14 in appendix iii. Many scientists have sought to characterise and classify liquid chromatography stationary phases in order to. Typically, 4 16 bed volumes are used in spe methods. Its extensive quality control procedure provides trouble free solid phase extraction methods. The highcapacity sorbent delivers maximum retention of anionic compounds, rounding out the companys complete offering for anioncation exchange separations. All file types, file format descriptions, and software programs listed on this page have been individually researched and verified by the fileinfo team. When searching for lowlevel biomarkers in the complex human plasma sample, researchers often push the sensitivity and detection limits of the lc separation and mass spectrometry platforms, but the effectiveness and robustness of the spe step is often overlooked.
Sensitive determination of polycyclic aromatic hydrocarbons. It is intended to ensure that spe protects data it maintains and assure the data are used only within the framework of the spe data use policy. Strata sorbent chemistries include a complete range of high performance silica and polymerbased nonpolar, polar and ionic phases that offer a wide range of diversity in addition to custom phases for specialized analysis. Sample preparation and hardware solutions for toxicology. A quick look at solid phase extraction spe solid phase extraction is an effective technique for cleaning up and concentrating samples, prior to hplc or gc analysis. The three most common extraction mechanisms used in spe are reversed phase, normal phase and ion exchange. The technique offers targeted selectivity for analytes of interest, versatility for a wide range of sample volumes, can be easily automated for highthroughput, and is performed.
Well do our best to solve the problem to your satisfaction. Solid phase extraction is a form of digital stepwise chromatography designed to extract, partition andor adsorb one or more components from a liquid phase sample onto stationary phase. Seven spe products from waters milford, ma with different sorbents andor sizes were compared in this study. This special dimension allows for a major improvement in sample throughput, while still providing great peak shapes and resolution. Tagged chemicals, fuels, jet fuel, liquid chromatography lc, luna, reproducibility. The manifold is supplied with a base and vacuum gauge, flask and stopper tubing and spigots. Develop a solid phase extraction spe method in under a minute.
We will address the different sample preparation techniques available for toxicology and clinical research. The patentpending design solves many of the common problems associated with column installation. Jul 01, 2012 solidphase extraction spe is often the method of choice for removing these salts. Strata silicabased solid phase extraction spe sorbents. Phenomenex hplc columns are warranted to meet the stated performance and quality and to be free of defects in material and workmanship. Phenomenex phenosphere sax 200 phenomenex luna nh 2 158 hamilton prp 2 shodex ic 220 antibiotics phenomenex gemini 126 phenomenex luna 158 phenomenex synergi 237 biotechnologylife sciences phenomenex clarity 300 phenomenex jupiter 300jupiter proteo 143 phenomenex onyx 185 phenomenex biosepsecs 95 phenomenex polysepgfcp 204 phenomenex. I have found the phenomenex zbsemivolatiles columns to. For each sample preparation technique, we will show how to apply it on specific methods and how it can be used to increase quality and throughput. Solid phase extraction spe protein precipitation ppt phospholipid removal plr supported liquid extraction.
The manifolds consist of a clear glass chamber and lid to which a vacuum is applied thereby drawing the sample. Ashmeeta chera, phenomenex sample preparation sales manager and matt brusius, phenomenex sample preparation product manager, discuss the major solid phase extraction faqs such as what is solid phase extraction spe. Intuitively we know more sorbent requires more solvent, less sorbent less solvent. Contact phenomenex for details regarding this method. Sample loading too fast allow sample to soak into the bed be.
Its low thermal mass means the coollock nut will be cool to the touch when the oven temperature is lowered, thus allowing you to change columns without fear of burning. These manifolds permit convenient batch processing of spe or sample filtration and help ensure consistent extraction and filtration results. The standard calibration curves were compared to the matrix spe extracts. Elute analyte product recommendation based on target analytes strong acids pk a spe products for your sample preparation needs. This guide gives you the starting point protocoles to help you develop your spe method depending on the sorbent youre using, your matrix and your analyte properties. To start, enter the name of the analyte youd like to extract or select proprietary to develop a method. Meticulous care is given to the quality control of surface smoothness, pore structure and. Increase reproducibility with the leakfree membrane.
Phenomenex now offers new optimized column dimensions 10 meter x 0. Clean extracts from biological sample matrices streamlined method development and simple processing stratax phase functional group mode analyte recommended alternative to waters stratax n n n n n o oh s oo o nh nh 2 o reversed phase polar and non. Lunas excellent silica is not simply the result of ultrapure metal free silica 99. Phenomenex catalog international 2009 nxtbook media.
Spe salvi encyclical letter of pope benedict xvi november 30, 2007 a digest by john gueguen introduction 1. Since then we developed the widest range of phases and products for spe. But what sort of hope could ever justify the statement that we are redeemed simply because of it. Make your lab more productive with our free, easytouse tools. We strive for 100% accuracy and only publish information about file formats that we have tested and validated. This concise diagram is designed to assist you in selecting. For a theoretical discussion of spe, and chromatographic theory in general, we suggest that the reader consult the literature references 1. We guarantee that if phenomenex products in this guide do not provide at least an equivalent separation as compared to other products of the same phase and dimensions, send in your comparative data within 45 days and keep the phenomenex product for free. Injection volume 1,800 l online spe, needle wash 3 seconds with acetonitrile autosampler temperature 18 c online spe enrichment column polaris 5 c18a, 3. Your introduction to solid phase extraction, how it works, and how to get started. In addition, the extended temperature stability range and. Learn how to develop a customized method for spe and view applications that are applicable to a variety of different industries.
Classification test for reversedphase hplc columns. Free sample pack of solid phase extraction spe tubes use our spe method development tool to quickly develop an spe method and request your free stratax sample pack. The complete guide to solid phase extraction spe phenomenex. Optimize your wash and elution sorbent wash and elution volumes the volume of solvent needed for spe processing is directly related to the mass of sorbent in the spe tube, and more specifically, the bed volume of the spe device.
Pdf breakthrough parameters of spe procedure on c18. This spe method development tool will recommend an extraction method for you in under 1 minute. With strata x polymeric spe sorbents the guesswork that leads to lengthy method development is eliminated. Unique selectivities have been developed to cover a diverse spectrum of analytes, and simplify the method development process for fast and efficient sample preparation. Solid phase extraction spe is a technique designed for rapid, selective sample preparation and purification prior to chromatographic analysis. The volume of and elution solvent silicaneeded for spe and elution processing is directly related to the mass of sorbent in the spe tube, and more specifically, the bed volume of the spe device. All strata spe phases and formats are the result of. If stratax spe products do not perform as well or better than your current spe. However, it should be noted that in spe, free silanols can often be used to.
Contact phenomenex for more information on product offerings and free samples. Chiral lc columns lux hplc columns tips for care and use. Stratax polymeric spe from phenomenex inc selectscience. Strata spe sample preparation products clean extracts. Strata spe products are manufactured by phenomenex to bring you optimum quality, reproducibility and performance. The use of underloaded c18 solidphase extraction plates. A simple approach to fast and practical solid phase extraction spe. Phenomenex solid phase extraction strata solid phenyl phase extraction material characteristics particle size pore size surface area carbon load bonding end capping ionic capacity phase m a m2g % meqg c18e 55 70 500 17. The resulting sample will have a significant amount of matrix effects removed. We use cookies to improve your experience and our website service. Solid phase extraction spe products for sample preparation.
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